This paper is to examine the cytogenetic effects (chromosom aberration and sister chromatid exchange) of the air pollutants at the areas of Sinchon-dong, Guro-dong, Banpodong and Jungnung-dong in Seoul from April to December 1987.
For this examination chromosomal aberrations and sister-chromatid exchanges were assayed using human peripheral blood lymphocytes. Samples of airborne particles were collected with high-volume air sampler using glassfiber filters. The sampling time lasted for 24hrs corresponding to a sampled air volume of 160o- 2400m3. The filters were extracted with benzene and ethanol mixture. Cytogenic effect test was performed with the total extract, which was quantitatively transferred to DMSO.
The results were as follows.
1. At the areas of Sinchon, Guro, Banpo and Jungnung the average SCE frequency were 10.6±2.4/cell, 10.9±2.9/cell, 12.9±3.0/cell, 8.9±2.9/cell respectively. SCE frequency of the airborne particles at the area of Banpo (express high way bus terminal) was higher than. that of other sampling, points. SCE frequency of negative control (DMSO) _ was--7 9+1 6/cell-.
2. The average SCE frequency of spring; -summer, autumn and winter were 10.9±2.5/ cell, 9.3±2.7/cell, 10.1±3.1/cell and 13.1±2.6/cell respectively.
3. The correlation coefficient(r) between airborne particle extracts and SCE frequency was 0.61.
4. The results of chromosomal aberration test in lymphocytes cultured for 52hrs with RPMI 1640-medium of winter airborne particle extracts were not detected.
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